![]() ![]() ΔΔCt method or standard curve method may be used.Į. ΔΔCt method or standard curve method will be used depending on the nature of mRNA.ĭevelop and validate RT-qPCR and/or ddPCR assays and test human bodily fluids for mRNA expression. The standard curve method will be used.ĭevelop and validate RT-qPCR and/or ddPCR assays and test human bodily fluids for mRNA expression. Recovery will be evaluated with spiked mRNA, and the RNA standard curve will be used to correct RT efficiency. Preclinical/Clinical Testing (GLP or non-GLP)ĭevelop and validate RT-qPCR and/or ddPCR assays and test tissues/blood from various animal models under GLP or non-GLP. Test potential endotoxin contamination using Limulus amebocyte lysate (LAL) method.ĭevelop or tech transfer custom assays from clients and validate/verify the assays for GMP testing. IVT in cell-free medium and detection of the resulting protein using Western blot/ELISA.ĭetermine mRNA/LNP encapsulation efficiency using RT-qPCR or ddPCR.ĭetect various dsRNAs using immunoblotting, native and denaturing gel electrophoresis.ĭevelop and validate a custom IVT assay to determine mRNA potency.ĭevelop and validate a custom assay to evaluate the long-term stability of mRNA. ![]() Quantify mRNA using UV Spectroscopy, RT-qPCR, or ddPCR. QPCR-based assay to detect potential plasmid contamination. Confirm the sequence of mRNA by sequencing cDNA by Sanger and NGS.ĭetermine mRNA size using CE, HPLC, Bioanalyzer, and/or northern blotting methods.ĭetermine the ratio of mRNA in a mixture using RT-qPCR or ddPCR. ![]()
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